Xiao Lehui

Xiao Lehui
Doctor degree Researcher 
Xiao Lehui, Doctor degree, Researcher, Research Center of Analytical Science,graduated from Hunan University
Special Talent:
Address:Room 209, College of Chemistry, Nankai University, Tianjin 
Research Interest:

1) single molecule detection

2) synthesis of functional nanomaterials for biomedical applications

3) nanophotonics


Full Professor Nankai University, Nankai, Tianjin, China 2016.04-now

Full Professor Hunan Normal University, Changsha, Hunan, China 2013.01-2016-03

Assistant Professor Hunan Normal University, Changsha, Hunan, China 2012.09-2013.01

Postdoc. Research University of Washington, Seattle, WA, U.S.A. 2011.12-2012.09

Postdoc. Research Ames Lab., DOE, Ames, Iowa, U.S.A. 2011.09-2011.12


Ph. D. Hunan University&Iowa State University 2008-2011 Analytical Chemistry

M. S. Hunan University&HongKong Baptist University 2006-2008 Analytical Chemistry

B. S. Hunan University 2002-2006 Chemistry

Honors and Awards


Henry Fok Young Teachers Award 2016 China

The National Science Fund for Excellent Young Scholars 2015 China

Program for New Century Excellent Talents in University 2014 China

The Hunan Province Science Fund for Distinguished Young Scholars 2014 Hunan Province

Award for Excellent Doctoral Student Granted by Ministry of Education 2010 China

Excellent Graduate Student 2010 Hunan University

Excellent Student 2005 Hunan University

2nd –Class Scholarship 2005 Hunan University

National Scholarship 2004 China

Triple A Student 2004 Hunan University

1st –Class Scholarship 2004 Hunan University

National Scholarship 2003 China

Triple A Student 2003 Hunan University

1st –Class Scholarship 

Scientific Achievements & Selected Publications

59 Xu, Y.; Li, Y.; Wei, L.; Liu, H.; Qiu, J.; Xiao, L.*, Attenuation of the Aggregation and Neurotoxicity of Amyloid Peptides with Neurotransmitter-Functionalized Ultra-Small-Sized Gold Nanoparticles. Eng. Sci., 2019, DOI: 10.30919/es8d778.

58 Zhao, Y.; Cai, J.; Liu, Z.; Li, Y.; Zheng, C.; Zheng, Y.; Chen, Q.; Chen, H.; Ma, F.; An, Y.; Xiao, L.; Jiang, C.; Shi, L.*; Kang, C.*; Liu, Y.*, Nanocomposites Inhibit the Formation, Mitigate the Neurotoxicity, and Facilitate the Removal of beta-Amyloid Aggregates in Alzheimer’s Disease Mice. Nano Lett. 2019, 19, 674-683.

57 Ye, Z.; Liu, H.; Wang, F.; Wang, X.; Wei, L.; Xiao, L.*, Single-particle tracking discloses binding-mediated rocking diffusion of rod-shaped biological particles on lipid membranes. Chem. Sci. 2019, 10, 1351-1359.

56 Ma, Y.; Wang, X.; Liu, H.; Wei, L.; Xiao, L.*, Recent advances in optical microscopic methods for single-particle tracking in biological samples. Anal. Bioanal. Chem. 2019. https://doi.org/10.1007/s00216-019-01638-z.

55 Liu, H.; Ye, Z.; Wang, X.; Wei, L.; Xiao, L.*, Molecular and living cell dynamic assays with optical microscopy imaging techniques. Analyst 2019, 144, 859-871.


54 Ye, Z.; Liu H.; Xiao L.* Recent Advances of Single Molecule/Particle Imaging with Optical Microscopic Methods. Sci. Sin. Chem. 2018, 48, https://doi.org/10.1360/N032018-00199. (Review, 庆祝南开大学成立100周年专刊)

53 Zhang, P.; Wang, H.; Zhang, D.; Zeng, X.; Zeng, R.*; Xiao, L.*; Tao, H.; Long, Y.; Yi, P.; Chen, J.*, Two-photon fluorescent probe for lysosome-targetable hypochlorous acid detection within living cells. Sens. Actuators B Chem. 2018, 255, 2223-2231.

52 Zhang, D.; Wei, L.; Zhong, M.; Xiao, L.*; Li, H.-W.; Wang, J., The morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy. Chem. Sci. 2018, 9, 5260-5269.

51 Yu, Y.; Ma, L.; Li, L.; Deng, Y.; Xu, L.; Liu, H.; Xiao, L.*; Su, X.*, Digestion of Dynamic Substrate by Exonuclease Reveals High Single-Mismatch Selectivity. Anal. Chem. 2018, 90, 13655-13662.

50 Ye, Z.; Weng, R.; Ma, Y.; Wang, F.; Liu, H.; Wei, L.*; Xiao, L.*, Label-Free, Single-Particle, Colorimetric Detection of Permanganate by GNPs@Ag Core-Shell Nanoparticles with Dark-Field Optical Microscopy. Anal. Chem. 2018, 90, 13044-13050.

49 Ye, Z.; Wei, L.; Zeng, X.; Weng, R.; Shi, X.; Wang, N.; Chen, L.; Xiao, L.*, Background-Free Imaging of a Viral Capsid Proteins Coated Anisotropic Nanoparticle on a Living Cell Membrane with Dark-Field Optical Microscopy. Anal. Chem. 2018, 90, 1177-1185.

48 Wei, L.; Zhang, D.; Zheng, X.; Zeng, X.; Zeng, Y.; Shi, X.; Su, X.; Xiao, L.*, Fabrication of Positively Charged Fluorescent Polymer Nanoparticles for Cell Imaging and Gene Delivery. Nanotheranostics 2018, 2, 157-167.

47 Wang, Y.-M.; Yang, Z.-R.; Xiao, L.; Yin, X.-B.*, Lab-on-MOFs: Color-Coded Multitarget Fluorescence Detection with White-Light Emitting Metal Organic Frameworks under Single Wavelength Excitation. Anal. Chem. 2018, 90, 5758-5763.

46 Shi, X.*; He, Y.; Gao, W.; Liu, X.; Ye, Z.; Liu, H.; Xiao, L.*, Quantifying the Degree of Aggregation from Fluorescent Dye-Conjugated DNA Probe by Single Molecule Photobleaching Technology for the Ultrasensitive Detection of Adenosine. Anal. Chem. 2018, 90, 3661-3665.

45 Russel, M.*; Sophocleous, M.; Shan, J.; Xu, W.; Xiao, L.; Maskow, T.; Alam, M.; Georgiou, J., High-frequency, dielectric spectroscopy for the detection of electrophysiological/biophysical differences in different bacteria types and concentrations. Anal. Chim. Acta 2018, 1028, 86-95.

44 Qi, F.; Han, Y.; Ye, Z.; Liu, H.; Wei, L.; Xiao, L.*, Color-Coded Single-Particle Pyrophosphate Assay with Dark-Field Optical Microscopy. Anal. Chem. 2018, 90, 11146-11153.

43 Pan, A.; Zhong, M.; Wu, H.; Peng, Y.; Xia, H.; Tang, Q.; Huang, Q.; Wei, L.; Xiao, L.*; Peng, C.*, Topical Application of Keratinocyte Growth Factor Conjugated Gold Nanoparticles Accelerate Wound Healing. Nanomedicine: NBM 2018, 14, 1619-1628.

42 Li, X.; Wei, L.; Pan, L.; Yi, Z.; Wang, X.; Ye, Z.; Xiao, L.*; Li, H.-W.*; Wang, J., Homogeneous Immunosorbent Assay Based on Single-Particle Enumeration Using Upconversion Nanoparticles for the Sensitive Detection of Cancer Biomarkers. Anal. Chem. 2018, 90, 4807-4814.

41 Deng, M.; Tang, Y.; Li, W.; Wang, X.; Zhang, R.; Zhang, X.; Zhao, X.; Liu, J.; Tang, C.; Liu, Z.; Huang, Y.; Peng, H.; Xiao, L.; Tang, D.; Scott, M. J.; Wang, Q.; Liu, J.; Xiao, X.; Watkins, S.; Li, J.; Yang, H.; Wang, H.; Chen, F.; Tracey, K. J.; Billiar, T. R.*; Lu, B.*, The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis. Immunity 2018, 49, 740-753.


40 Zhong, W.; Zeng, X.; Chen, J.*; Hong, Y.; Xiao, L.; Zhang, P.*, Photoswitchable fluorescent polymeric nanoparticles for rewritable fluorescence patterning and intracellular dual-color imaging with AIE-based fluorogens as FRET donors. Polym. Chem. 2017, 8, 4849-4855.

39 Zhang, Z.; Zhang, D.; Wei, L.; Wang, X.; Xu, Y.; Li, H.-W.; Ma, M.*; Chen, B.; Xiao, L.*, Temperature responsive fluorescent polymer nanoparticles (TRFNPs) for cellular imaging and controlled releasing of drug to living cells. Colloid. Surface. B. 2017, 159, 905-912.

38 Wei, L.; Ma, Y.; Zhu, X.; Xu, J.; Wang, Y.; Duan, H.; Xiao, L.*, Sub-diffraction-limit localization imaging of a plasmonic nanoparticle pair with wavelength resolved dark-field microscopy. Nanoscale 2017, 9, 8747-8755.

37 Wei, L.; Ma, Y.; Shi, X.; Wang, Y.; Su, X.; Yu, C.; Xiang, S.; Xiao, L.*; Chen, B.*, Living cell intracellular temperature imaging with biocompatible dye-conjugated carbon dots. J. Mater. Chem. B 2017, 5, 3383-3390.

36 Wang, N.; Zhang, Y.; Lei, X.; Yu, W.; Zhan, Y.; Wang, D.; Zhang, J.; Wang, A.; Xiao, L.; Jiang, P.*; Yang, Y.*, Optimized conditions for preserving stability and integrity of porcine circovirus type2 virus-like particles during long-term storage. J. Virol. Methods 2017, 243, 146-150.

35 Wang, J.; Xiong, G.; Ma, L.; Wang, S.; Zhou, X.; Wang, L.; Xiao, L.; Su, X.*; Yu, C.*, A dynamic sandwich assay on magnetic beads for selective detection of single-nucleotide mutations at room temperature. Biosens. Bioelectron. 2017, 94, 305-311.

34 Tan, H.; Huang, Y.; Xu, J.; Chen, B.; Zhang, P.; Ye, Z.; Liang, S.; Xiao, L.*; Liu, Z.*, Spider Toxin Peptide Lycosin-I Functionalized Gold Nanoparticles for in vivo Tumor Targeting and Therapy. Theranostics 2017, 7, 3168-3178.

33 Su, X.; Li, Z.; Yan, X.; Wang, L.; Zhou, X.; Wei, L.; Xiao, L.*; Yu, C.*, Telomerase Activity Detection with Amplification-Free Single Molecule Stochastic Binding Assay. Anal. Chem. 2017, 89, 3576-3582.

32 Song, W.; Duan, W.; Liu, Y.; Ye, Z.; Chen, Y.; Chen, H.; Qi, S.; Wu, J.; Liu, D.; Xiao, L.*; Ren, C.*; Chen, X., Ratiometric Detection of Intracellular Lysine and pH with One-Pot Synthesized Dual Emissive Carbon Dots. Anal. Chem. 2017, 89, 13626-13633.


31 Yang, Q.; Ye, Z.; Zhong, M.; Chen, B.; Chen, J.; Zeng, R.; Wei, L.*; Li, H.-W.*; Xiao, L.*, Self-Assembled Fluorescent Bovine Serum Albumin Nanoprobes for Ratiometric pH Measurement inside Living Cells. ACS Appl. Mater. Interfaces 2016, 8, 9629-9634.

30 Wei, L.; Ye, Z.; Xu, Y.; Chen, B.; Yeung, E. S.; Xiao, L.*, Single Particle Tracking of Peptides-Modified Nanocargo on Lipid Membrane Revealing Bulk-Mediated Diffusion. Anal. Chem. 2016, 88, 11973-11977.

29 Wei, L.; Ye, Z.; Luo, W.; Chen, B.; Xiao, L.*, Redistribution of fluorescent molecules at the solid/liquid interface with total internal reflection illumination. Talanta 2016, 155, 229-234.

28 Wei, L.; Xu, J.; Ye, Z.; Zhu, X.; Zhong, M.; Luo, W.; Chen, B.; Duan, H.; Liu, Q.; Xiao, L.*, Orientational Imaging of a Single Gold Nanorod at the Liquid/Solid Interface with Polarized Evanescent Field Illumination. Anal. Chem. 2016, 88, 1995-1999.

27 Tan, H.; Luo, W.; Wei, L.; Chen, B.; Li, W.; Xiao, L.*; Manzhos, S.*; Liu, Z.*; Liang, S., Quantifying the Distribution of the Stoichiometric Composition of Anticancer Peptide Lycosin-I on the Lipid Membrane with Single Molecule Spectroscopy. J. Phys. Chem. B 2016, 120, 3081-3088.

26 Poon, C.-Y.; Wei, L.; Xu, Y.; Chen, B.; Xiao, L.*; Li, H.-W.*, Quantification of Cancer Biomarkers in Serum Using Scattering-Based Quantitative Single Particle Intensity Measurement with a Dark-Field Microscope. Anal. Chem. 2016, 88, 8849-8856.

25 Ma, Y.; Zhang, Z.; Xu, Y.; Ma, M.; Chen, B.; Wei, L.*; Xiao, L.*, A bright carbon-dot-based fluorescent probe for selective and sensitive detection of mercury ions. Talanta 2016, 161, 476-481.

24 Luo, W.; Wu, M.; Li, S.; Xu, Y.; Ye, Z.; Wei, L.; Chen, B.; Xu, Q.-H.; Xiao, L.*, Nanoprecipitation of Fluorescent Conjugated Polymer onto the Surface of Plasmonic Nanoparticle for Fluorescence/Dark-Field Dual-Modality Single Particle Imaging. Anal. Chem. 2016, 88, 6827-6835.


23 Yang, Q.; Wei, L.; Zheng, X.; Xiao, L.*, Single Particle Dynamic Imaging and Fe3+ Sensing with Bright Carbon Dots Derived from Bovine Serum Albumin Proteins. Sci. Rep. 2015, 5.


22 Xiao, L.*; Yeung, E. S.*, Optical Imaging of Individual Plasmonic Nanoparticles in Biological Samples. Ann. Rev. Anal. Chem. 2014, 7, 89-111.

21 Wei, L.; Zhou, P.; Yang, Q.; Yang, Q.; Ma, M.; Chen, B.; Xiao, L.*, Fabrication of bright and small size semiconducting polymer nanoparticles for cellular labelling and single particle tracking. Nanoscale 2014, 6, 11351-11358.

20 Wei, L.; Yang, Q.; Xiao, L.*, Tempo-spatially resolved cellular dynamics of human immunodeficiency virus transacting activator of transcription (Tat) peptide-modified nanocargos in living cells. Nanoscale 2014, 6, 10207-10215.

19 Li, H.; Guo, Y.; Xiao, L.*; Chen, B.*, Selective and sensitive detection of acetylcholinesterase activity using denatured protein-protected gold nanoclusters as a label-free probe. Analyst 2014, 139, 285-289.

18 Li, H.; Guo, Y.; Xiao, L.*; Chen, B.*, A fluorometric biosensor based on H2O2-sensitive nanoclusters for the detection of acetylcholine. Biosens. Bioelectron. 2014, 59, 289-292.


17 Xiong, X.; Yang, Z.; Li, Y.; Xiao, L.; Jiang, L.; Chen, Y.; Ma, M.; Chen, B.*, Preparation of a polyhedral oligomeric silsesquioxane-based perfluorinated monolithic column. J. Chromatogr. A. 2013, 1304, 85-91.

16 Wei, L.; Zhao, X.; Chen, B.; Li, H.; Xiao, L.*; Yeung, E. S.*, Frozen Translational and Rotational Motion of Human Immunodeficiency Virus Transacting Activator of Transcription Peptide-Modified Nanocargo on Neutral Lipid Bilayer. Anal. Chem. 2013, 85, 5169-5175.

15 Wei, L.; Liu, C.; Chen, B.; Zhou, P.; Li, H.; Xiao, L.*; Yeung, E. S.*, Probing Single-Molecule Fluorescence Spectral Modulation within Individual Hotspots with Subdiffraction-Limit Image Resolution. Anal. Chem. 2013, 85, 3789-3793.

14 Sun, W.; Xiao, L.; Fang, N.*, Imaging non-fluorescent nanoparticles in living cells with wavelength-dependent differential interference contrast microscopy and planar illumination microscopy. Methods Mol. Biol. 2013, 931, 169-186.

13 Li, Y.; Xiao, L.-H.; Zhou, N.-Y.; Yuan, P.; Wu, Y.-N.*; Chen, B.*, Purification of Pigments by Iron Oxide Nanoparticles for Analysis of Pesticide Residues in Tea. Chinese J. Anal. Chem. 2013, 41, 63-68.

+++++++++++++++++++++++++Independent Work+++++++++++++++++++++++++++


12 Xiao, L.; Wei, L.; Liu, C.; He, Y.; Yeung, E. S.*, Unsynchronized Translational and Rotational Diffusion of Nanocargo on a Living Cell Membrane. Angew. Chem. Int. Ed. 2012, 51, 4181-4184.

11 Xiao, L.; Ha, J. W.; Wei, L.; Wang, G.; Fang, N.*, Determining the Full Three-Dimensional Orientation of Single Anisotropic Nanoparticles by Differential Interference Contrast Microscopy. Angew. Chem. Int. Ed. 2012, 51, 7734-7738.

10 Chan, H.-M.; Xiao, L.; Yeung, K.-M.; Ho, S.-L.; Zhao, D.; Chan, W.-H.; Li, H.-W.*, Effect of surface-functionalized nanoparticles on the elongation phase of beta-amyloid (1-40) fibrillogenesis. Biomaterials 2012, 33, 4443-4450.


9 Xiao, L.; Wei, L.; Cheng, X.; He, Y.*; Yeung, E. S., Noise-Free Dual-Wavelength Difference Imaging of Plasmonic Resonant Nanoparticles in Living Cells. Anal. Chem. 2011, 83, 7340-7347.

8 Xiao, L.; Qiao, Y.; He, Y.*; Yeung, E. S., Imaging Translational and Rotational Diffusion of Single Anisotropic Nanoparticles with Planar Illumination Microscopy. J. Am. Chem. Soc. 2011, 133, 10638-10645.

7 Wei, L.; Xiao, L.; He, Y.*, Synthesis of water soluble silver-nanoparticle-embedded polymer nanofibers with poly(2-ethyl-2-oxazoline) by a straightforward polyol process. J. Mater. Res. 2011, 26, 1614-1620.

6 Sun, W.; Xiao, L.; Fang, N. Cell Imaging Techniques: Methods and Protocols, Methods in Molecular Biology, Human Press. Springer, Book Chapter, 2011.


5 Xiao, L.; Zhao, D.; Chan, W.-H.; Choi, M. M. F.; Li, H.-W.*, Inhibition of beta 1-40 amyloid fibrillation with N-acetyl-L-cysteine capped quantum dots. Biomaterials 2010, 31, 91-98.

4 Xiao, L.; Wei, L.; He, Y.*; Yeung, E. S., Single Molecule Biosensing Using Color Coded Plasmon Resonant Metal Nanoparticles. Anal. Chem. 2010, 82, 6308-6314.

3 Xiao, L.; Qiao, Y.; He, Y.*; Yeung, E. S.*, Three Dimensional Orientational Imaging of Nanoparticles with Darkfield Microscopy. Anal. Chem. 2010, 82, 5268-5274.


2 Xiao, L.; Zhou, R.; He, Y.*; Li, Y.; Yeung, E. S.*, Direct Observation of Nanoparticle Self-Assembly Dynamics at the Water-Air Interface Using Differential Interference Contrast Microscopy. J. Phys. Chem. C 2009, 113, 1209-1216.

1 Xiao, L.; He, Y.*; Yeung, E. S.*, High Throughput Single Molecule Spectral Imaging of Photoactivated Luminescent Silver Clusters on Silver Island Films. J. Phys. Chem. C 2009, 113, 5991-5997.

Professional Cultivation